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Release August 26, Date Added January 23, Version Operating Systems. Total Downloads 22, Downloads Last Week 0. Report Software. Related Software. Desktop Lighter Free. Adjust the brightness of your computer screen. Intel Graphics Driver for Windows Free. Because the data file is a repository of all information about the data, its size will change every time it is reprocessed.

As of Star Version 6. Instead, a version of the results and data handling method is added to the data file and older calculations can be accessed. Method Files The Method File file with a. MTH extension contains all of the instrument and data handling method sections required to process a set of samples. While most sections are only modified when edited by the user, a method may also contain sections that are modified under automation.

Such sections contain 'historical' information that extends in scope beyond a single run, for example: Blank Baseline: The data from a designated sample is reduced to simplified baseline information and stored in the method, to be subtracted during the data handling from data files subsequently processed by the same method.

The blank baseline information is incorporated into each data file as it is processed, along with the rest of the data handling method. Calibration: During a calibration, the method is updated based on new calibration data points, building the calibration curve one point at the time. Again, the calibration information is incorporated into data files as they are processed.

Methods can be password-protected the password is controlled by the security application, which is itself password-protected.

Methods can contain revision notes, and the system can be set- up via the security application to require a revision entry to be made when the method is changed. Therefore, older versions of the method can be accessed. The service of the data generators i. As a result, data acquisition will be reliable even in a significantly overloaded system.

If the computer became so busy that some of the data generators could not be serviced in a timely manner, the system would report error messages and shut down. No incorrect data can be produced as a result of an overload. The data from a given data generator is saved into a single file, which can have one or more channels of data. After the data acquisition is completed, post-run processes are automatically performed, such as Peak Processing and the printing of Reports.

Peak Processing Peak Processing transforms raw chromatographic data into useful results. It is controlled by the data handling section of the data file method, and is performed automatically at the end of every chromatographic run. Raw data can also be recalculated later in System Control or Interactive Graphics with a different method to adjust the processing parameters or focus on a different aspect of the data. The information used to recalculate the results is then saved in the data handling section of the data file method.

The first section of this manual is devoted to a discussion of data handling fundamentals. The main method parameters affecting the peak detection are the Noise Options, the Peak Width settings, and the Signal-to- Noise ratio. Noise Options The noise is monitored prior to the start of each run. The Monitor Length in the Detector Information Window determines the number of data points to be acquired, and indicates the total length of the monitoring period.

At the end of the data acquisition, a noise value is computed and stored in the data file for each channel of data acquired. Savitzky and M. Golay, Anal. Note that it is possible for your instrument to report a zero noise reading. This may happen if you use a high bunch rate setting, or if a low gain is used on a detector connected to an ADCB in which case, both signal and noise are decreased, and the noise may not be measurable.

Peak Width Setting The width of chromatographic peaks vary greatly based on experimental conditions, and may also vary as a function of the retention time in a given experiment. To accommodate these variations, the data points stored in the data file may be further bunched averaged by the peak processing software so that the current peak width setting translates into a number of bunched points between 10 and 20 the bunch factor is a power of 2.

This bunching process affects the measured slope and it is important that the width setting be the right order of magnitude. If the Time Events table does not specify a peak width setting WI event , the software will start with a peak width of 4 seconds and automatically update the peak width and the corresponding bunch rates based on the measured width of the peaks encountered.

If peak width settings are specified, no automatic width update will occur. Instead, the software will use the specified time-programmed width values and deduce the corresponding bunch rates. In either case, width updates will not occur during a peak, and will only occur after the end of a peak. Exceeding this threshold indicates the onset of a peak. The following is a synopsis of the detection of an isolated peak.

The last 60 bunched points are examined backwards to find a local minimum, which is then taken as the Peak Start. Peak Apex A peak apex is found when the slope becomes negative. The time of the apex is determined by a linear interpolation between the last point with a positive slope and the first point with a negative slope. The amplitude of the apex is determined as the highest of the two corresponding bunched data points.

Calculation of Peak Size The Star Workstation will calculate peak size in terms of peak area, peak height, or square root of peak height.

Calculation of Peak Area Peak Areas are computed by adding the areas of trapezoids created by dropping perpendiculars from each data point to the zero value. Then, the area of the trapezoid comprised between the integration baseline and the zero line is subtracted. You have the option of reporting the peak height or the square root of the peak height.

The LSB represents the smallest data increment that can be measured. Starting with Version 6. An acceptable error on the peak area should be equal to the peak to peak noise multiplied by the time difference between peak end and peak start. A simple way to verify that Peak Areas are globally correct is to compare the peak area to the theoretical value of 1. This formula is not very accurate if the peak is lost in the noise or is asymmetrical.

Subtract Blank Baseline If you choose, you can have the Star Workstation subtract a blank baseline from the chromatogram data. This allows you to correct for reproducible baseline drift over the chromatogram, which can result from temperature programming in GC analyses or elution gradient programming in LC analyses.

In the report, both the chromatogram and the results reflect this correction. In most cases, peak heights and areas should not be significantly affected by the use of a blank baseline. Peak Identification Peaks in a chromatogram are mapped to compound names by means of the peak table contained in the method.

Identification of each compound is based on its retention time. Replicates and Calibration Levels One or more concentration levels are used to define a calibration curve, and the Star Workstation supports the use of up to 10 levels. Within each level, multiple replicates can be used. The Star Workstation offers two options for the treatment of replicates. Average Replicates: Each level is represented by one 'historical' value. Each new replicate is averaged in with the historical value with a method defined weight to produce a new historical value.

Keep Replicates Separate: Each replicate within the level is kept as a separate data point. Up to ten replicates per level are allowed.

The calibration curve and the calculations will include all replicates. The weight for point i will be noted wi in the rest of this manual. The Star Workstation uses the least squares method to determine the coefficients of the equation which approximates the calibration curve. You can calculate the coefficients for your calibration equation independently, using an Excel worksheet or by hand. If you use an Excel worksheet, bear in mind that Excel may display a negligible exponential value rather than zero when numbers are displayed in the 'General' format.

If appropriate, you can solve this problem by selecting the 'Numeric' format. The three options are to Ignore, Include, or Force the origin. Include: Add the origin to the data set. Force: Force the curve to go through the origin. This means that the coefficient a0 is null. For the determination of peak purity, the varian prostar module varian, palo alto, ca, usa with prostar solvent delivery and a prostar hplc-dad was used.

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